青蒿中青蒿素的提取分离研究

Study on abstraction and separating of artemisinin in Artemisia annus L

对青蒿中青蒿素进行了不同溶剂分离提取的试验,结果表明:于50℃恒温回流提取2次,固液比为1:10/1:8,时间为2h/1h的条件下,石油醚(30-60℃)为最佳溶媒介质,1%活性炭脱色的效果最佳,80%甲醇洗提取液的浓缩浸膏得到进一步分离纯化。此工艺提取分离所得的青蒿素的结晶经HPLC法测定其含量最高为70.761%。

This text artemisinin in the sweet wormwood,decolours,the methyl alcohol,ethanol of different density have the medicinal extract,and set up thin layer and scan the law and high-efficient liquid phase and measure the law.The result indicate that on 50 thermostatical backset current drawing 2,firm liquid than 1:10 / 1:8,time is under terms of 2h/1h,In order to dissolve the media quality the petroleum ether(30 degrees Centigrade-60 degrees Centigrade) bestly,the result that 1% of the active charcoal decolours is best,80% of the methyl alcohol washes and concentrates the medicinal extract to receive and separate the purification further.In order to dissolve the media quality the petroleum ether(30 degrees Centigrade-60 degrees Centigrade) bestly,the result that 1% of the active charcoal decolours is best,80% of the methyl alcohol washes and concentrates the medicinal extract to receive and separate the purification further.Craft this draw thick crystallization of artemisinin to separate income determine by HPLC law his supreme content up to 70.761%.