卵巢肿瘤血清VEGF水平和肿瘤组织中MVD的检测

Detection of Serum VEGF Level and Expression of MVD in Ovarian Neoplasm Tissue

目的:探讨卵巢肿瘤血清血管内皮生长因子(VEGF)水平和肿瘤组织中微血管密度(MVD)的表达与其临床病理的关系。方法:对45例卵巢恶性肿瘤、18例卵巢良性肿瘤及12例正常卵巢对照采用ELISA法检测血清VEGF的水平以及LAB免疫组织化学法测定其MVD。结果:①恶性肿瘤患者血清VEGF水平明显高于良性肿瘤和正常卵巢组织(P<0.05);上皮性癌中晚期癌(Ⅲ期、Ⅳ期)的血清VEGF水平明显高于早期癌(Ⅰ期、Ⅱ期);②恶性肿瘤卵巢组织中MVD明显高于良性肿瘤和正常卵巢组织(P<0.05)。结论:VEGF和MVD可能参与卵巢恶性肿瘤的演化过程,对判断卵巢肿瘤良恶性程度有一定临床意义;血清VEGF可能成为上皮性卵巢癌一种新的肿瘤标记物。

Objective:To investigate the serum level of vascular endothelial growth factor(VEGF) in patients with ovarian neoplasm and the expression of microvessel density(MVD) in ovarian neoplasm tissue;to evaluate the relationship with the clinicopathologic factors.Methods:The serum VEGF of 75 patients were detected by enzymelinked immunosorbent assay;including 45 ovarian malignant tumors,18 benign ovarian tumors and 12 normal ovaries.Their MVD expressions were analyzed by immunohistochemical methods.Results:①The serum VEGF levels in malignant ovarian tumors were higher than those of benign ovarian tumors and normal ovarian tissues(P<0.05);the serum VEGF levels of late stages(Ⅲ、Ⅳ) in epithelial ovarian cancer were higher than those of early stages(Ⅰ、Ⅱ).②The expressions of MVD in malignant ovarian tumors were higher than those of benign ovarian tumors and normal ovaries(P<0.05).Conclusion:VEGF and MVD may be play an important role in the angiogenic progression of malignant ovarian tumors;They have some extent clinical significances in diagnosis of ovarian neoplasms.