天麻多糖GEP-2对BCG+LPS致小鼠免疫性肝损伤的保护作用

Protection of polysaccharide GEP-2 from gastrodia elata on the immunity-mediated liver injury induced by BCG+LPS in mice

目的:观察天麻多糖GEP-2对卡介苗加脂多糖(BCG+LPS)致小鼠免疫性肝损伤的影响。方法:制备BCG+LPS致小鼠免疫性肝损伤模型,比色法测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的含量,肝脏中超氧化物歧化酶(SOD)活力、丙二醛(MDA)浓度以及谷胱甘肽还原酶(GSH-Px)活力,酶联免疫法测定血清TNF-α、IL-1的含量,用MTT法测定脾T、B淋巴细胞增殖能力。结果:天麻多糖GEP-2(25、50、100mg/kg)能明显降低小鼠血清中升高的ALT和AST水平以及TNF-α、IL-1的含量,抑制肝脏中上升的MDA水平和提高过低的SOD、GSH-Px活性,且各用药组均能提升脾T、B淋巴细胞增殖能力。结论:天麻多糖GEP-2对BCG+LPS致小鼠免疫性肝损伤具有显著的保护作用。

Objective:To observe the protection of polysaccharide GEP-2 from Gastrodia elata on the immunity-mediated liver injury induced by BCG+LPS in mice.Methods:BCG and LPS were adopted to establish BCG+LPS liver injury model in mice. The contents of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), the activity of superoxide dismutase (SOD),glutathion reductase (GSH-Px) and malondiadehyde (MDA) consistent of liver homogenate in mice were measured by colorimetric method. The content of tumor necrosis factor-α(TNF-α)and interleukin-1( IL-1)in serum were measured by enzyme linked immunosorbent assay (ELISA), and T-and B-lymphocyte proliferation were measured by MTT.Results:Polysaccharide GEP-2 from Gastrodia elata (25,50,100 mg/kg) could obviously reduce the high level of ALT and AST,and serum TNF-α and IL-1 either.It inhibited high level of MDA, increased the low activity of SOD and GSH-Px in liver homogenate,and enhanced T-and B-lymphocyte proliferation.Conclusion:Polysaccharide GEP-2 from Gastrodia elata had apparently protective effects on the immunity liver injury in mice induced by BCG+LPS.