FGF1和PD98059对成年大鼠肝细胞FGFR2表达的影响

The influence of FGF1 and PD98059 on FGFR2 expression in vitro on the membrane of the cultured primary rat hepatocytes

在体外培养过程中观察生长因子FGF1和MEK特异性阻滞剂PD98059二者对原代培养的成年SD大鼠肝细胞表面与FGF1结合的胞膜受体FGFR2表达量的影响。采用两步法原位灌注取肝细胞,培养到一定时间,加FGF1和PD98059,继续培养一段时间后流式细胞仪检测细胞增殖周期比例和受体FGFR2的表达。对照组和实验组肝细胞,均大量的表达FGFR2,表达量之间没有差异,均在90%左右。FGF1和PD98059对成年SD大鼠肝细胞表面FGFR2的表达没有反馈调节及阻抑作用。

To observe the influence of FGF1 and PD98059 on FGFR2 expression in vitro on the membrane of the cultured primary rat hepatocytes.Hepatocytes were isolated by two-step collagenase perfusion in situ and FGF1.And PD98059 were added into the medium.After cultured for some time,the distribution of the cell cycle and the expression of the FGFR2 were analyzed by Flow Cytometer.FGFR2 was expressed on the membrane of about 90 percent of the hepatocytes whether freshly isolated by two-step collagenase perfusion of the liver in situ or cultured in vitro with or without FGF1 and FD98059.There was no difference between different groups of the hepatocytes.FGF1 and PD98059 didn't show any feedback or inhibition of the FGFR2 expression.