IL-10基因多态性与乙肝肝硬化易感性的相关性研究

Association of the genetic polymorphisms of interleukin(IL)-10 with susceptibility to HBV-induced liver cirrhosis

探讨白细胞介素10(IL-10)基因多态性与乙型肝炎肝硬化易感性的关系.采用聚合酶链反应-限制性片段长度多态性(PCR-PFLP)分析方法,检测100例乙肝肝硬化患者及124例健康对照组IL-10基因启动子-1082G/A、-592A/C位点的基因多态性,并确定了其基因型和等位基因频率的分布.肝硬化组与对照组IL-10基因启动子-592A/C位点基因型分布频率和等位基因频率差异无显著性(P＞0.1),肝硬化组-1082G/A位点AA基因型频率及A等位基因频率高于对照组(P＜0.05),G等位基因相对于A等位基因患肝硬化的机会比为0.373(95%CI:0.166～0.838).IL-10基因多态性与肝硬化易感性相关,基因启动子-1082G/A位点AA基因型携带者肝硬化易感性高.

To study the possible association of the genetic polymorphism of interleukin(IL)-10 with the susceptibility to HBV-induced liver cirrhosis.Peripheral blood samples were collected from 100 patients with HBV-induced liver cirrhosis and 124 healthy controls.The polymorphisms at position-1082G/A,-592A/C of the promoter of IL-10 gene were determined by polymerase chain reaction-restriction fragment length polymorphisms analysis(PCR-RFLP).For position-592A/C,the genotype frequency and allele frequencies were not significantly different between the cirrhosis group and healthy group(P>0.1).For position-1081G/A,relative higher AA genotype frequency and an A allele frequency were found in cirrhosis group(P<0.05).And the rate to get liver cirrhosis was 0.373 among people with G allele compared with A allele(95%confidence interval was 0.116-0.838).The results suggested that the genotype of IL-10-1082G/A positiion was associated with the susceptibility to HBV-induced liver cirrhosis.