抗-HCV-IgG阳性血清检测HCV的临床意义

The clinical values for the definitive detection of HCV in several clinical cases such as positive HCV-IgG

了解抗-HCV-IgG阳性时、仅肝功能轻度异常、与HCV感染者有接触史时等临床情况下HCV在血液内存的几率,验证在这些临床情况时开展检测HCV确证试验的临床意义。采集血清为检测标本,用ELISA技术检测抗-HCV-IgG,RT-PCR技术检测HCV-RNA。血清HCV-RNA的检出率,抗-HCV-IgG阳性组为41.9%、仅肝功能轻度异常组为25.7%、无症状体检组为29.4%。临床对抗-HCV-IgG阳性者、仅肝功能轻度异常者、有接触史者等特殊人群应做检测HCV的确证试验,RT-PCR技术可以用做临床检测HCV的确证试验方法。

To understand the detected probability of hepatitis C virus(HCV) in blood in several clinical cases such as positive HCV-IgG,light abnormal liver profile and the possibility for HCV of contagion.In order to appraise the clinical values for the definitive test was used to detect HCV in the clinical cases.HCV-IgG was detected by enzyme-linked immunosorbent assay(ELISA) and HCV-RNA was detected by reverse transcription-polymerase chain reaction(RT-PCR) in serum.The positive rate of HCV-RNA in the serum with RT-PCR were 41.9% in the patients with positive HCV-IgG,25.7% in the patients with light abnormal liver profile and 29.4% in the patients with the possibility for HCV of contagion.There are the positive results of HCV-RNA in serum with RT-PCR in the clinical cases on the study.In the clinical cases,the paitents ought to do a definitive test to detect HCV.RT-PCR is a very useful tool for clinical definitive detection of HCV.