肝癌组织中p27蛋白表达与肿瘤增殖凋亡活性的研究

Effect of the expression of p27 on cell proliferation and apoptosis in hepatocellular carcinoma

研究肝细胞癌组织中p27表达对细胞增殖与凋亡活性的影响。采用E livision法检测47例肝癌及相应癌旁肝组织中p27及PCNA的表达,TUNEL法检测原位细胞凋亡。结果显示肝癌组织中细胞增殖与凋亡指数明显高于癌旁肝组织。肝癌组织中p27染色指数明显低于癌旁肝组织与正常肝组织。低分化肝癌组织中p27表达和凋亡指数均明显降低。存在肝外转移的肝癌组织中p27表达降低而增殖指数明显增高。进一步研究显示,p27高表达组肝癌组织中凋亡指数明显高于低表达组,而增殖指数却显著降低。提示p27蛋白表达降低与肝癌的发生和进展有着密切关系,并可能是导致肝癌细胞增殖凋亡失衡的因素之一。

To investigate the effects of p27 on the cell proliferation and apoptosis in hepatocellular carcinoma(HCC).The expression of p27 and proliferating cell nuclear antigen(PCNA) was measured by immunohistochemical technique in cancer samples and paracancer tissues from 47 patients with HCC.And cell apoptosis was detected by TUNEL.The proliferatin index(P1) and apoptotic index(AI) in HCC tissues were significantly higher than that in paracancer tissues.The p27 labeling index(LI) was significantly lower in HCC than that in paracancer liver tissues or normal liver tissue.The p27 LI and AI in HCC tissues with low differentiation were significantly lower than those in HCC tissues with well differentiation.The p27 LI was significantly decreased in cases with extrahepatic metastasis.While the PI was just on the contrary.It suggested that the decreasing of p27 expression in HCC ws closely associated with HCC progression and metastasis,and might be involved in the imbalance of proliferation and apoptosis in HCC.