摘要
目的:了解体外培养的人脂肪间充质干细胞的生物学特性及其体外成脂和成骨的能力。方法:体外培养人脂肪间充质干细胞并传代计数,行成骨和成脂诱导,流式细胞仪检测其细胞增殖周期与表面分子。结果:人脂肪干细胞经过体外培养均一的阳性表达CD44、CD106,而CD49d、CD34、CD45和HLA-DR表达阴性。细胞周期分析表明:G0/G1、S和G2/M所占比例分别为79.1%、19.7%和1.3%。分离细胞在诱导体系下可以向成骨和成脂方向分化。结论:脂肪间充质干细胞具有特殊的生物学特点,体外能够向成骨和成脂方向分化。
Objective To investigate the biological characteristics of mesenchymal stem cells from human adipose tissue.Methods Adipose stem cells were highly purified and culture expanded The morphology,phenotype and biological properties of the cultured adipose stem cells were observed by flow cytometer.Results Adipose stem cells possessed some unique phenotypes.Adipose stem cell were positive of CD44、 CD106 and negative of CD49d、CD34、CD45 和HLA-DR.Cell cycle studies showed there were percentage of stem cells which G0 /G179.1%、S 19.7 and G2 /M 1.3% respectively.After 7days of adipocyte inducing,about 85%of the cells displayed accumulation of lipid vacuoles,as detected by Red Oil O.After 14 days of osteogenic inducing,ALP stains were positive.Conclusion Adipose stem cell from adult human can be induced to differentiate into adipocytes and osteoblast.
出处
《中国美容医学》
CAS
2007年第2期159-162,共4页
Chinese Journal of Aesthetic Medicine
基金
全军"十一五"医学科研面上项目(编号:06M0I8)
