RP-HPLC法测定软肝颗粒中原儿茶醛的含量

Determination of protocatechualdehyde in Ruan Gan Granules by RP-HPLC

目的 采用反相高效液相色谱法测定软肝颗粒中原儿茶醛的含量.方法 采用十八烷基硅烷键合硅胶柱;流动相:甲醇-1%醋酸溶液(12∶88),检测波长为279nm;流速为1.0mL·min-1.结果 原儿茶醛线性范围0.0552～0.2208μg,平均回收率为99.9%,RSD=0.5%.结论 方法简便,结果准确,能有效控制软肝颗粒的质量.

OBJECTIVE To determine the content of protocatechualdehyde in Ruan Gan Granules by RP-HPLC.METHODS The determination was carried out with Symmetry C18column.The mobile phase consisted of CH3OH-1% acetic acid(12∶88).The flow rate was 1mL·min-1 and the detective wavelength was 279nm.RESULTS The content of protocatechualdehyde can be determined by RP-HPLC.The linear concentration ranges of these compound were 0.0552～0.2208μg.The average recoveries were 99.9%,RSD was 0.5%.CONCLUSION The method was simple and accurate and can be used to control the quality of Ruan Gan Granules.