β受体阻滞剂对动脉粥样硬化兔体内氧化应激的影响

Effects of β-blockers on the oxidative stress in the atherosclerotic rabbit

目的:对比观察β受体阻滞剂卡维地洛、普萘洛尔与阿替洛尔对动脉粥样硬化家兔体内氧化应激的影响。方法:将24只雄兔随机分为高脂对照组(HC组)、高脂加卡维地洛(10mg.kg-1.d-1)组(CAR组)、高脂加普萘洛尔(10mg.kg-1.d-1)组(PRO组)和高脂加阿替洛尔(20mg.kg-1.d-1)组(ATE组)。各组给予相应处理1周后,行腹主动脉球囊损伤术,并继续相应处理10周。另6只家兔给予正常饮食并施以假手术作为正常对照(NC)组。实验进程中动态观察血清脂质过氧化物(LPO)和超氧化物歧化物(SOD)的变化。实验结束时取胸主动脉标本,超氯化物荧光探针染色检测血管内原位超氧化物的生成。结果:与NC组比较,HC组血清LPO水平明显升高,SOD水平明显降低,血管内原位超氧化物生成均明显升高(均P<0.05)。CAR组明显降低血清脂质过氧化物44.3%、升高SOD活性1.3倍,并明显降低血管内原位超氧化物生成(均P<0.05),而PRO与ATE组均未对上述指标产生明显影响。结论:与普萘洛尔和阿替洛尔相比,卡维地洛有抗动脉粥样硬化氧化应激的作用。

Objective:To compare the effects of carvedilol,propranolol and atenolol on the oxidative stress in the atherosclerotic rabbit.Method:Rabbits were randomized into 4 groups(n=6):hyperlipidemic diet only(hyperlipidemic control,HC)or hyperlipidemic diet supplemented with propranolol(10 mg·kg-1·d-1),atenolol(20 mg·kg-1·d-1),or carvedilol(10 mg·kg-1·d-1).After treated for 1 week,they underwent balloon injuries and remained their respective diets for the further 10 weeks.6 sham untreated rabbits received normal diets(normal control,NC).At the end of the trial,LPO level and SOD content in serum were tested.Dihydroethidium staining for superoxide in aortic segments were also measured.Result:In HC group,LPO level in serum increased significantly(P<0.01).While LPO level was markedly reduced by 44.3% and SOD level was increased by 1.3-fold in carvedilol group,which was not treated with propranolol or atenolol.In carvedilol group but not propranolol and atenolol,rterial superoxide levels was significantly reduced.Conclusion:The study suggests that more effect of carvedilol in preventing development of atherosclerosis may be related to antioxidant activity.These beneficial effects of carvedilol could not be shared by other β-blockers such as propranolol or atenolol.