摘要
本文介绍傅立叶变换表面等离子共振技术(FT-SPR)的基本原理和关键技术的特点。以下体系的实时研究证明,在检测分子相互作用中,新的FT平台提供非常高的灵敏度和很宽的测定范围,如醇和水共溶体、DNA杂化、抗体-肽结合。FT-SPR技术为生命和材料科学的研究人员提供新的可能,他们不仅可以监控分子的结合过程,而且可以了解过程的化学本质。
In this paper,we introduce the basic principles and key technical features of Fourier Transform Surface Plasmon Resonance(FT-SPR) technique.The ultra high sensitivity and large dynamic range offered by the new FT platform in detecting molecular interactions are demonstrated with real-time studies of the following interacting systems: EtOH and water co-solvent;DNA hybridization;antibody-peptide binding.FT-SPR opens a new door for researchers in the field of life and materials sciences not only to monitor the binding processes,but also to unveil the chemistry behind these processes.
出处
《现代仪器》
2006年第5期10-13,共4页
Modern Instruments
