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海藻糖酶的提取及其酶学性质的研究 被引量：1

The Purification of Trehalase and the Research of Character
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摘要 由家蚕通过酶分离纯化得到海藻糖酶的活力单位为25U/mL,计算得海藻糖酶的米氏常数Km为27.8mmol/L,最适温度为45oC,最适pH为5.4.由pH稳定性实验可知海藻糖酶在pH4.0～7.0范围内相对稳定,在pH＜4时,酶活力显著降低.通过海藻糖酶的热稳定性实验可以看出该酶热稳定性良好.实验还确定了反应的最低酶量,初步探讨了海藻糖酶分解海藻糖的过程.
作者 王春辉 金利群 郑裕国
出处 《中国食品学报》 EI CAS CSCD 2003年第z1期6-9,共4页 Journal of Chinese Institute Of Food Science and Technology
基金 国家自然基金资助项目(No.20176055)浙江省自然基金资助项目(No.2B0106)
关键词 海藻糖酶 分离纯化 家蚕
分类号 TS2 [轻工技术与工程—食品科学与工程]
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参考文献7

  • 1[1]Joelma Freire de Mesquita,Vania M.Flosi Paschoalin,Anita D.Panek.Modulation of trehalase activity in Saccharomyces cerevisiae by an intrinsic protein [J].Biochimica et Biophysica Acta,1997,1334:233～239
  • 2[2]Thomas J.Oesterreicher,Diane C.Markesich,Susan J.Henning,Cloning.characterization and mapping of the mouse trehalase (Treh) gene [J].Gene,2001,270:211～220
  • 3[3]Algimantas P.Valaitis,Diana F.Bowers.Purification and properties of the soluble midgut trehalase from the Gypsy moth,Lymantria dispar [J] .Biochem.Molec.Biol.1993,23 (5):599～606
  • 4[4]Murray I.A.,Coupland K.,Smith J.A.,Anser I.D.,Long,R.G.Intestinal trehalase activity in a UK population:establishing a normal range and the effect of disease [J] .Br.J.Nutr.,2000,83:241 ～245
  • 5[5]Sumida M,Ogura S,Miyata S,Arai M,Murao S.Purification and some properties of trehalase from Chaetomium aureum MS- 27[J] .J.Ferment Bioeng,1989,67:83～86
  • 6[6]Svetlana V.Kyosseva,Zhetcho N.Kyossev,Alan D.Elbein.Inhibitors of Pig Kindey Trehalase [ J ].Archives of Biochemistry and Biophysics,1995,316(2) :821～826
  • 7[7]B.Keshan,A.K.Ray.Subcellular localization of trehalase in different tissues of the silkworm,Bombyx mori L.:Demonstration of the highest activity in mitochondria [ J ].Sericologia,1998,411～416

同被引文献20

  • 1雷芳,张桂芬,万方浩,马骏.寄主转换对B型烟粉虱和温室粉虱海藻糖含量和海藻糖酶活性的影响[J].中国农业科学,2006,39(7):1387-1394. 被引量：30
  • 2王荫长.昆虫生物化学.北京:中国农业出版社,2004.240～ 247.
  • 3Alaoui A, Gourdoux 1,, Atay ZK, Moreau R, 1994. Alterations in carbohydrate metabolism induced in Locusta migratoria after poisoning with the pyrethroid insecticide deltamethrin. Pestic. Biochem. Physiol. , 50 ( 3 ) : 183 -190.
  • 4Becker A, Schl：der P, Steele JE, Wegener G, 1996. The regulation of trehalose metabolism in insects. Experientia, 52(5) :433 -439.
  • 5Bradford MM, 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. , 72:248 - 254.
  • 6Chang PL, Morrison PE, 1975. Histochemical localization of trehalase activity in dorsal flight muscle of the flesh fly Sarcophaga bullata with light and electron microscopy. J. Histochem. Cytochem. , 23 (10) :800 - 807.
  • 7Lee JH, Tsuji M, Nakamura M, Nishimoto M, Okuyama M, Mori H, Kimura A, Matsui H, Chiba S, 2001. Purification and identification of the essential ionizable groups of honeybee, Apis mellifera L. , trehalase. Biosci. Biotechnol. Biochem. , 65(12) :2657 -2665.
  • 8Nath BS, 2003. Shifts in glycogen metabolism in hemolymph and fat body of the silkworm, Bombyx mori ( Lepidoptera: Bombycidae) in response to organophosphorus insecticides toxicity. Pestic. Biochem. Physiol., 74:73-84.
  • 9Ogiso M, Takahashi SY, 1984. Trehalases from the male accessory glands of the Americanco-ckroach:developmental changes and the hormonal regulation of the enzymes. Gen. Comp. Endocrinol. , 55:387 - 392.
  • 10Park N J, Lim HK, Hwang IT, 2008. An enhanced system to screen trehalase inhihitors using a microplate assay with housefly enzyme source. J. Asia-Pac. Entomol. , 11 (3) : 161 - 166.

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中国食品学报
2003年 第z1期

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