雷公藤在大鼠1型糖尿病模型中对调节性T细胞Foxp3表达的影响及意义

Significance and effect of Glucosidorum Tripterygii tororum on expressions of Foxp3 in regulatory T cells in type 1 diabetic rat model

目的:研究雷公藤在大鼠1型糖尿病模型中对CD4+CD25+调节性T细胞(Tr)Foxp3基因表达的影响及意义。方法:将48只大鼠随机分为3组,每组各16只,Ⅱ、Ⅲ组采用小剂量多次腹腔注射链脲佐菌素(STZ)的方法制备大鼠1型糖尿病模型,Ⅰ组为正常对照。Ⅲ组在成模后给与雷公藤多甙(GTT)灌胃,1次/d,连续3个月。淋巴细胞转化试验(MTT法)检测脾淋巴细胞增殖活性;荧光定量PCR检测外周血及脾淋巴细胞Foxp3 mRNA的表达;免疫组化检测淋巴结和脾组织FOXP3蛋白的表达。结果:Ⅱ、Ⅲ组血糖水平高于Ⅰ组(P<0.01);胰岛中Ⅲ组淋巴细胞浸润程度较Ⅱ组减轻;Ⅱ、Ⅲ组淋巴细胞增殖活性均较Ⅰ组升高(P<0.01),用药后Ⅲ组较Ⅱ组降低;Foxp3 mRNA、FOXP3蛋白表达水平Ⅱ、Ⅲ组均高于Ⅰ组(P<0.05),Ⅲ组表达水平较Ⅱ组有所升高,但差别无统计学意义(P>0.05)。结论:Foxp3+Tr细胞参与了STZ诱导的大鼠1型糖尿病的发生发展;上调Tr细胞Foxp3基因的表达可能为雷公藤治疗1型糖尿病的机制之一。

AIM:To investigate the significance and effect of Glucosidorum Tripterygii tororum(GTT)on the expressions of Foxp3 in CD4+CD25+ regulatory T cells(Tr)in type 1 diabetic rat model.METHODS:48 rats were divided evenly into 3 groups by random.Diabetic model was developed by multiple low dose intraperitoneal injection of streptozotocin in groupⅡ,Ⅲ,and groupⅠwas treated as normal control.After the establishment of the model,administration of GTT was conducted in group Ⅲ,which was performed once a day,lasting thre...