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149位丝氨酸在CDC25B诱导小鼠卵母细胞减数分裂中的功能 被引量：3

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摘要 目的:研究小鼠卵母细胞减数分裂过程中,149位丝氨酸对CDC25B磷酸酶功能的影响。方法:将野生型CDC25B-WT和突变型CDC25B-S149A体外转录成mRNA,显微注射到小鼠GV期卵母细胞中,观察减数分裂恢复情况,同时检测了各组卵母细胞CDC2-Tyr15的磷酸化状态。结果:在小鼠卵母细胞减数分裂过程中,野生型CDC25B能促进减数分裂的重启动,活化有丝分裂促进因子,其突变体CDC25B-S149A对减数分裂的诱导作用丧失,不能活化有丝分裂促进因子。结论:149位丝氨酸的完整是CDC25B磷酸酶发挥调节功能所必需的。
作者 赵鸿梅 张阳 徐晓燕 于秉治
机构地区 中国医科大学生物化学教研室 中国医科大学基因组教研室
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2008年第3期298-299,302,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 高等学校博士学科点专项科研基金资助(20050159019)
关键词 卵母细胞 减数分裂 GVBD CDC25B
分类号 Q25 [生物学—细胞生物学]
  • 相关文献

参考文献7

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二级参考文献15

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共引文献13

同被引文献43

  • 1张阳,张杰,于爱鸣,宗志红,于秉治.蛋白激酶A/Cdc25B通路在小鼠卵母细胞G_2期阻滞中作用的研究[J].生殖与避孕,2005,25(4):195-200. 被引量：14
  • 2Lobjois V,Jullien D,Bouche JP,et al.The polo-like kinase1 regulates CDC25B-dependent mitosis entry.BiochimBiophys Acta,2009,1793(3):462-8.
  • 3Strausfeld U,Labbe JC,Fesquet D,et al.Dephosphory-lation and activation of a p34cdc2/cyclin B complex invitro by human CDC25 protein.Nature,1991,351(6323):242-5.
  • 4Lincoln AJ,Wickramasinghe D,Stein P,et al.Cdc25bphosphatase is required for resumption of meiosis dur-ing oocyte maturation.Nat Genet,2002,30(4):446-9.
  • 5Duncan FE,Moss SB,Williams CJ.Knockdown of thecAMP-dependent protein kinase(PKA)type Ialpha regu-latory subunit in mouse oocytes disrupts meiotic arrestand results in meiotic spindle defects.Dev Dyn,2006,235(11):2961-8.
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引证文献3

二级引证文献7

细胞与分子免疫学杂志
2008年 第3期

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