三种淋球菌培养基分离效果对比分析

Contrastive analysis of isolating effect of three gonococcus culture medium

目的评价新型淋球菌培养基的分离效果。方法将我院研制的新型淋球菌培养基(实验组)与英国的Oxoid公司生产的TM培养基(TM组)、国产MTM培养基(MTM组)对20株淋球菌进行分离培养对比分析。结果实验组菌落数最多,18、24小时与其它两组之间的差异有显著性意义(P<0·05),36、48小时与TM组之间的差异有显著性意义(P<0·05);实验组、TM组和MTM组内18小时与24小时菌落数差异有显著性意义(P<0·05)。MTM组24小时与36小时菌落数差异也有显著性意义(P<0·05));18小时TM组菌落较小,与其它两组比较差异有显著性意义(P<0·05)。24小时TM组菌落较小,与MTM组比较差异有显著性意义(P<0·05)。36小时实验组菌落较小,与其它两组比较差异有显著性意义(P<0·05)。48小时实验组菌落较小,与MTM组比较差异有显著性意义(P<0·05);3组菌落大小均随时间增长而增大,各时间段间菌落大小的差异有显著性意义(P<0·05)。结论新型淋球菌选择培养基分离淋球菌具有经济、简便、灵敏度高等优点,是一种较理想的培养基。

ObjectiveTo determine the effect of isolating N.gonococcus by a new style selective medium. MethodsThe new style medium made by our hospital (experimental group) together with Oxoid’s TM selective medium (TM group) and domestic-made MTM selective medium (MTM group) were applied to culturing 20 strains of N.gonococcus. ResultsThe experimental group which showed significant difference with other two groups at 18h and 24h, and with TM at 36h and 48h(P < 0.05)had more colonies. Each medium had significant di...