摘要
探讨B7-H3分子对人外周血单核细胞来源树突状细胞(Mo-DC)体外成熟和生物学功能的影响。采用常规方法从健康人外周血单核细胞诱导DC,在诱导过程中,加入B7-H3单抗21D4共培养,经流式细胞术检测Mo-DC上B7-H3分子和其他共刺激分子的表达,ELISA试剂盒检测培养上清中细胞因子IL-10和IFN-γ的分泌量,并采用3H-TdR掺入法测定T细胞的增殖。结果:B7-H3分子在未成熟和成熟Mo-DC上均有高水平表达,抗人B7-H3单抗21D4能上调Mo-DC表面CD80、CD86和CD83的表达,提高Mo-DC的共刺激能力,促进T细胞的体外增殖,并能显著促进T细胞分泌IL-10。由此表明,B7-H3单抗21D4交联作用可以促进Mo-DC体外成熟,上调其共刺激T细胞的能力。
To investigate the effect of the B7-H3 molecule on maturation and biological functions of the peripheral blood monocyte derived dendritic cells(Mo-DC) in vitro,the human Mo-DC were co-cultivated with monoclonal antibody against B7-H3 molecule 21D4 in the course of induction,and the expressions of B7-H3 and other co-stimulatory molecules on Mo-DC were analyzed by flow cytometry(FCM).Meanwhile,the amounts of secretion in cytokines IL-10 and IFN-γ in the cultural supernatants were determined by ELISA and the 3...
出处
《现代免疫学》
CAS
CSCD
北大核心
2008年第2期116-120,共5页
Current Immunology
基金
国家自然科学基金重点资助项目(30330540)
江苏省高校自然科学基础研究项目(QZ134610)