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B7-H3单抗交联作用对Mo-DC体外生物学功能的影响 被引量：5

Effect of cross-linking of the monoclonal antibody against B7-H3 molecule on the in votro biological functions of the peripheral blood monocyte derived dendritic cells
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摘要 探讨B7-H3分子对人外周血单核细胞来源树突状细胞(Mo-DC)体外成熟和生物学功能的影响。采用常规方法从健康人外周血单核细胞诱导DC,在诱导过程中,加入B7-H3单抗21D4共培养,经流式细胞术检测Mo-DC上B7-H3分子和其他共刺激分子的表达,ELISA试剂盒检测培养上清中细胞因子IL-10和IFN-γ的分泌量,并采用3H-TdR掺入法测定T细胞的增殖。结果:B7-H3分子在未成熟和成熟Mo-DC上均有高水平表达,抗人B7-H3单抗21D4能上调Mo-DC表面CD80、CD86和CD83的表达,提高Mo-DC的共刺激能力,促进T细胞的体外增殖,并能显著促进T细胞分泌IL-10。由此表明,B7-H3单抗21D4交联作用可以促进Mo-DC体外成熟,上调其共刺激T细胞的能力。 To investigate the effect of the B7-H3 molecule on maturation and biological functions of the peripheral blood monocyte derived dendritic cells(Mo-DC) in vitro,the human Mo-DC were co-cultivated with monoclonal antibody against B7-H3 molecule 21D4 in the course of induction,and the expressions of B7-H3 and other co-stimulatory molecules on Mo-DC were analyzed by flow cytometry(FCM).Meanwhile,the amounts of secretion in cytokines IL-10 and IFN-γ in the cultural supernatants were determined by ELISA and the 3...
作者 朱一蓓 张光波 周迎会 李文香 胡益洲 张学光
机构地区 苏州大学生物技术研究所 苏州大学附属第一医院临床免疫学实验室
出处 《现代免疫学》 CAS CSCD 北大核心 2008年第2期116-120,共5页 Current Immunology
基金 国家自然科学基金重点资助项目(30330540) 江苏省高校自然科学基础研究项目(QZ134610)
关键词 树突状细胞 B7-H3 抗体交联作用 dendritic cells B7-H3 cross linking of the mAb
分类号 R392.12 [医药卫生—免疫学]
  • 相关文献

参考文献13

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二级参考文献11

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共引文献8

同被引文献33

  • 1胡玲玲,徐耀瑜,陈永井,马泓冰,程钢,刘玉华,王艳茹,孙杰,陈明心,邱玉华.抗人CD86人-鼠嵌合抗体的构建及在CHO细胞的表达[J].现代免疫学,2008,28(2):104-109. 被引量：6
  • 2陈成,朱一蓓,周桓,瞿秋霞,王勤,马倩茹,胡玉敏,张学光.CD40配基化调节小鼠树突状细胞上B7-H3分子表达的研究[J].中华微生物学和免疫学杂志,2006,26(3):228-231. 被引量：3
  • 3闫峰,蔡莉,惠延年,王雨生,孟浩.TGFβ2-DC对小鼠同种异体角膜移植排斥反应的抑制作用[J].国际眼科杂志,2007,7(2):346-349. 被引量：4
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引证文献5

二级引证文献14

现代免疫学
2008年 第2期

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