反复自然流产患者外周血调节性T细胞Foxp3的表达

Expression of mRNA and protein in the transcription factor Foxp3 in peripheral regulatory T cells of women with unexplained recurrent miscarriage

评价原因不明复发性流产(unexplained recurrent miscarriage,URM)患者外周血CD4+CD25+调节性T细胞中Foxp3的表达。以正常生育非孕妇女(n=20)为对照,以URM(n=25)为研究对象,采用实时荧光定量PCR分析外周血单个核细胞Foxp3转录水平;以流式细胞术分析外周血CD4+CD25+T细胞Foxp3翻译水平。结果:实时荧光定量PCR显示,URM患者的Foxp3转录水平明显低于正常对照组(P<0.01)。流式细胞仪分析显示URM组外周血中CD4+CD25+T细胞Foxp3翻译水平显著低于正常对照组(P<0.01)。URM患者外周血调节性T细胞Foxp3表达降调可能参与URM的发病。

To evaluate the significance of the expression of the transcription factor Foxp3 in peripheral regulatory T cells(Treg) of women with unexplained recurrent miscarriage(URM),25 cases of women with URM were underwent for study and 20 normal non-pregnant women were used as control.The expression level of the transcription factor Foxp3 mRNA in the peripheral blood nucleated cells was determined by real-time fluorescence quantitative PCR,and the translation level in CD4+CD25+ Treg of the peripheral blood was ass...