标本放置时间和温度对血清乳酸脱氢酶活性的影响

Study on the Effect of Activity of Serum Lactic Dehydtogenase in Blood Specimens During Ambient Temperature and Time

目的:分析血标本在不同放置时间和温度对血清乳酸脱氢酶(LD)的影响。方法:采用34例健康成人血标本分别放置于4℃～6℃、23℃～25℃和37℃,在即时、8 h、24 h、48 h、72 h和96 h测定其血清LD活性并进行分析比较。结果:在4℃～6℃条件下,分别于即时和放置8 h、24 h、48 h、72 h及96 h测定血清LD活性结果为(127.2±8.7)U/L、(125.5±12.6)U/L、(130.2±14.2)U/L、(145.8±18.4)U/L、(161.3±24.7)U/L和(191.2±28.0)U/L.经统计学分析,标本放置8 h和24 h与即时测定结果无明显差异(P>0.05),当标本放置48 h、72 h和96 h血清LD活性比即时测定结果明显升高(P<0.05、P<0.001);当标本放置24 h,冷藏组(4℃～6℃)、室温组(23℃～25℃)和温育组(37℃)血标本LD活性分别为(130.2±14.2)U/L、(141.9±15.1)U/L和(168.8±17.1)U/L,与即时测定结果比较,冷藏组(P>0.05)无明显差异,而室温组(P<0.05)和温育组(P<0.01)血清LD活性明显升高。结论:随着血标本放置时间的延长和放置温度的增加血清LD活性会逐渐升高。

Objective To analyze the effect to activity of lactic dehydrogenase (LD) in blood specimens during ambient temperature and time.Methods Activity of serum LD of the blood samples from 34 health adults was determined in 4℃～6℃,23℃～25℃and 37℃when immediate 0 h,8 h,24 h,48 h,72 h and 96 h.Results When immediate 0 h,8 h,24 h,48 h,72 h and 96 h,activity of serum lactic dehydrogenase was respectively (127.2±8.7) U/L,(125.5±12.6) U/L,(130.2±14.2)U/L,(145.8±18.4)U/L,(161.3±24.7)U/L and (191.2±28.0)U/L In 4℃～6℃.In 4℃～...