高效液相色谱法测定人血清中5-HT含量

To Detect 5-HT in Serum of Humans with HPLC Method

目的:建立以高效液相色谱法测定人血清中5-羟色胺(5-HT)含量的方法。方法:色谱柱为SinoChromODS-BP柱(250mm×4.6mmID,5μm0.05,大连依利特),流动相为0.05mol/L KH2PO4:甲醇=95∶5(磷酸调pH至4.0),柱温为40℃,检测波长为275nm,流速为1.0ml/min。结果:5-HT检测进样量在0.01μg^1.6μg范围内线性关系良好(r=0.9999),平均回收率为84.4%(RSD=1.096%,n=5)。结论:本方法调整了流动相的比例,调整了5-HT的保留时间。与文献报道方法相比,减少了杂质峰的干扰,更加简便、准确、重复性好,故可用于血清中5-HT含量测定。

Objective To establish a HPLC method for determiniation of 5-HT in serum of humans.Methods The separation column,SinoChrom ODS-BP column(250 mm×4.6 mm ID,5 μm0.05 dalian);mobile phase ,0.05mol/L kalium dihydrogen phosphate methanol(95:5),the pH of mobile phase was adjusted to reach 4.0 with orthophosphoric acid; colume temperature ,40℃;detecting wavelength,275 nm;flowrate,1.0ml.min-1.Results The calibration curve of 5-HT was linear in the concentration range of 0.01 μg^1.6 μg(r=0.9999),the average recovery ...