生物催化立体选择性氧化还原中存在问题及其发展策略

Biocatalytic systems for stereoselective oxidoreduction: existing limitations and development strategies

以立体选择性氧化还原酶或其全细胞催化的不对称氧化还原反应已经成为转化光学活性手性醇及其他手性化合物的有效手段。然而,生物催化氧化还原反应体系存在着催化活性与专一性、反应体系与催化稳定性等生物催化剂所固有的局限性问题,而且,生物氧化还原反应必需辅酶及其再生问题也是限制该转化途径产业化应用的一个重要因素。围绕上述生物催化立体选择性氧化还原中存在的关键问题,现代分子生物技术及反应工程的不断突破和发展为改善生物催化立体选择性氧化还原在催化剂本身和反应工程方面的局限性提供了有效的发展策略,为其进一步大规模产业应用提供了发展基础。

Stereoselective oxidoreductions catalyzed by enzymes or whole cells have been widely used for preparation of optically active alcohols and other chiral compounds.However,perceived and real limitations of biocatalysts,including limited enzyme availability,reaction scope,and operational stability,still occur to biocatalytic oxidoreductions.In addition,the necessity of cofactors and their regeneration in biooxidoreduction is also a significant limitation of biocatalytic redox for industrial application.For the...