盾叶薯蓣悬浮培养细胞生长及薯蓣皂苷元合成

Diosgenin biosynthesis in suspension cultures of Dioscorea zingiberensis cells

研究了盾叶薯蓣细胞悬浮培养过程中细胞生长、薯蓣皂苷元合成、蔗糖和磷酸盐的吸收利用以及酸性磷酸酶活性与薯蓣皂苷元合成的关系。结果表明,对数生长期细胞最大比生长速率μm为0.19 d-1;倍增时间为3.68 d;薯蓣皂苷元的形成与细胞的生长相关,培养6 d时薯蓣皂苷元质量分数和产量分别为0.20%和25.93 mg/L;蔗糖利用率达到95.65%,磷酸盐吸收率达到最大,为90.36%。盾叶薯蓣细胞悬浮培养过程中酸性磷酸酶活性动态变化规律与薯蓣皂苷元的动态合成规律基本一致。此外,研究还发现在相同供磷水平下,酸性磷酸酶活性高低与薯蓣皂苷元合成能力呈正相关;而在不同供磷水平下,酸性磷酸酶活性高低与薯蓣皂苷元合成能力没有相关性。

Growth,diosgenin biosynthesis,the consumotion of sucrose and inorganic phosphate in the medium,as well as the relationship of acid phosphatase activity and diosgenin biosynthesis in D.zingiberensis cell suspension cultures were studied.The results show that the maximal specific growth rate was 0.19 d-1and double time was 3.68 d in the exponential phase.The intensity of diosgenin biosynthesis was correlated to cell growth.On the 6th day of cultivation,the content and yield of diosgenin reached the maximal le...