摘要
目的研究1-磷酸鞘氨醇(S1P)在肺组织修复过程中的作用及其机制。方法用1、10μMS1P及其受体激动剂SEW2871处理人胚胎肺纤维细胞,分别将10μM细胞外信号调节激酶(ERK)拮抗剂PD98059、磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)拮抗剂LY294002与1μMS1P共同处理人胚胎肺纤维细胞48h,观察对细胞增殖的影响;采用Western blot法研究信号传导通路。结果S1P1μM和10μM处理组细胞增殖明显增加,S1P1受体激动剂也明显促进人胚胎肺纤维细胞增殖,Westernblot结果显示S1P和SEW2871处理过的细胞磷酸化ERK和Akt表达增加,ERK及PI3K/Akt抑制剂可有效抑制S1P引起的细胞增殖作用。结论S1P与S1P1受体结合并通过ERK1/2和PI3K/Akt通路引起人胚胎肺纤维细胞增殖;S1P通过引起纤维细胞的增殖反应可能参与肺损伤的修复过程。
Objective To study the effect and mechanism of S1P on the lung tissue remodeling.Methods HFL-1 cultured with S1P(1 μM,10 μM)and S1P1 agonist(SEW2871)to assess the proliferation of the cells.S1P 1μM together with ERK antagonist(PD98059,10 μM),PI3K/Akt antagonist(LY294002,10 μM),treated cells for 48 hours and the proliferation effect was detected.The signal transduction pathway was studied by western blot.Results S1P(1 μM,10 μM)and S1P1 agonist increased HFL-1 proliferation significantly.Western blot results ...
出处
《山东医药》
CAS
北大核心
2008年第5期15-17,共3页
Shandong Medical Journal
关键词
细胞增殖
1-磷酸鞘氨醇
信号传导
proliferation
sphingosine 1-phosphate
signal transduction
