气升式反应器中生物脱硫过程工艺因素的研究

Technical Parameters of Dibenzothiophene Biodesulfurization in an Air-Lift Bioreactor

利用已分离所得的具有高脱硫活性的微杆菌ZD-M2,以正十六烷为模拟油相,二苯并噻吩(DBT)为模型含硫化合物,在自行设计的气升式反应器中进行模拟燃料油生物脱硫的研究。通过与摇床的对比实验,确定不同休止细胞浓度下反应器中最佳比脱硫速率为0.283mmol·(kgDCW)-1·min-1,是摇床实验最佳比脱硫速率的2.26倍;进一步确定实验条件下的最适空塔气速为7.07cm·s-1,并考察了不同生长介质和DBT浓度对生物脱硫的影响。结果显示过高气量下氧的传质速率反而降低,影响脱硫效果;ZD-M2休止细胞在生长介质(培养基)和非生长介质(磷酸缓冲溶液和生理盐水)中具有几乎相同的脱硫活性;较高的休止细胞浓度和DBT浓度都不利于传质的进行;推断ZD-M2降解正十六烷中DBT发生在有机相-水相界面。

Using hexadecane as model oil and dibenzothiophene (DBT) as model sulphocompound, the simulating study on oil biodesulfurization was conducted by the biodesulfurization of DBT in an airlift bioreactor with Microbacterium sp. ZD-M2, which has the capability of converting DBT into 2-hydroxybiphenyl(2-HBP). The experimental results show that, for the used airlift bioreactor, the most suitable empty column gas velocity is 7.07 cm·s-1, and under different resting cell concentrations, the highest specific desulfu...