摘要
目的探讨二氢嘧啶脱氢酶(DPD)在胃癌组织中的表达和临床意义。方法以-βactin为内参照,应用半定量RT-PCR技术检测51例T3或T4化疗前胃癌组织中DPD的mRNA水平。结果以DPD mRNA表达水平的中位数(0.68)为界,将患者分为高表达组和低表达组。DPD mRNA高表达组(>0.68)和低表达组(≤0.68)之间总生存期、无瘤生存期及1、2年生存率差异均有统计学意义(P<0.05);而DPD mRNA表达与年龄、性别、淋巴结转移及组织学分级均无相关性(均P>0.05)。结论检测DPD mRNA表达水平可预测胃癌患者的预后。
Objective To study the expression level of dihydropyrimidine dehydrogenase(DPD) mRNA in gastric cancer tissues and its clinical significance.Methods Expression level of DPD mRNA in 51 cases of gastric adenocarcinoma tissues was detected by reverse transcription polymerase chain reaction(RT-PCR) and β-actin was used as a internal reference gene.Relative amount of DPD mRNA was expressed as the ratio of DPD to β-actin RT-PCR products.Results Median value,0.68 of DPD mRNA,was selected for cut-off value,which se...
出处
《苏州大学学报（医学版）》
CAS
北大核心
2008年第1期65-67,共3页
Suzhou University Journal of Medical Science
基金
无锡市科技局科研项目(CS055012)
关键词
胃肿瘤
DPD
预后
gastric neoplasms
dihydropyrimidine dehydrogenase
prognosis
