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塞来昔布对子宫内膜癌细胞株HEC-1-B生长及其VEGF表达的影响

Inhibitory Effect of Celecoxib on Endometrial Carcinoma Cell Line and the Expression of VEGF
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摘要 目的研究塞来昔布对子宫内膜癌细胞株HEC-1-B的生长及其血管内皮生长因子(VEGF)表达的影响。方法应用不同浓度的塞来昔布作用于HEC-1-B细胞,MTT法检测塞来昔布作用于HEC-1-B细胞24、48、72h后,对HEC-1-B细胞增殖的影响。用逆转录聚合酶链反应(RT-PCR)分析其对HEC-1-B细胞VEGF mRNA表达的影响,同时用酶联免疫吸附实验(ELISA)检测HEC-1-B细胞上清中VEGF蛋白表达量。结果塞来昔布对HEC-1-B细胞的生长有抑制作用,且呈剂量和时间依赖性;与对照组相比,塞来昔布能降低HEC-1-B细胞VEGF mRNA及蛋白的表达。结论塞来昔布能抑制HEC-1-B细胞的生长及VEGF的分泌。 Objective To explore the effect of celecoxib on cell proliferation and the expression of vascular endothelian growth factor(VEGF) in human endometrial carcinomar cell line.Methods HEC-1-B cells were cultured in MEM199 medium supplied with different concentration of celecoxib.The proliferative activity of HEC-1-B cells was evaluated by MTT assay.The expression of VEGF in the cells was determined by RT-PCR and enzyme-linked immunosorbent assay(ELISA).Results Cell proliferation was inhibitated in time-dependen...
作者 宋明旭 李莉华 黄朝晖 刘志辉 周希科
机构地区 苏州大学附属第四医院中心实验室
出处 《苏州大学学报（医学版）》 CAS 北大核心 2008年第1期62-64,共3页 Suzhou University Journal of Medical Science
关键词 塞来昔布 子宫内膜癌 血管内皮生长因子 celecoxib endometrial carcinoma HEC-1-B cell VEGF
分类号 R737.33 [医药卫生—肿瘤]
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参考文献5

  • 1李淞漪,帅茨霞,林亚.环氧合酶-2及其抑制剂与妇科肿瘤相关性研究进展[J].国外医学（妇产科学分册）,2004,31(6):362-365. 被引量：1
  • 2[2]Subbaramaiah K,Zakim D,WekslerB B,et al.Inhibition of cyclooxy genase:a novel approach to cancer prevention[J].Proc Soc Exp BioMed,1997,216(2):201-210.
  • 3[3]Sheng HM,Shao JY,Morrow JD.Modulation of apoptosis and bcl-2 expression by prostaglandin E2 in human colonic cancer cells[J].Cancer Res,1998,58(7):362-366.
  • 4[4]Yokoyama Y,Sato S,Futagami,et al.Prognostic significance of vascular endothelian growth factor and its receptors in endometrial carcinomar[J].Gyneeol Oncol,2000,77(3):413-418.
  • 5[5]Masferrer JL,Leahy KM,Koki AT,et al.Anti anglogenie and antitumor activities of cyclooxygenase-2 inhibitors[J].J Cancer Res,2000,60(5):1306-1311.

二级参考文献20

  • 1Denkert C, Kobel M, Pest S,et al.Expression of cyclooxygenase 2 is an independent prognostic factor in human ovarian carcinoma.Am J Pathol,2002,160(3):893-903.
  • 2Seo SS, Song YS, Kang DH, et al. Expression of cyclooxygenase-2 in association with clinicopathological prognostic factors and molecular markers in epithelial ovarian cancer. Gynecol Oncol,2004,92(3):927-935.
  • 3Li S, Miner K, Fannin R, et al. Cyclooxygenase- 1 and 2 in normal and malignant human ovarian epithelium. Gynecol Oncol,2004,92(2):622-627.
  • 4Ali-Fehmi R, Che M, Khalifeh I, et al. The effect of cyclooxygenase-2 expression on Tumor vascularity in advanced stage ovarian serious carcinoma. Cancer,2003,98(7): 1423-1429.
  • 5Denkert C, Weichert W, Pest S, et al. Overexpression of the embryoniclethal abnormal vision-like protein HuR in ovarian carcinoma is a prognostic factor and is associated with increased cyclooxygenase 2 expression.Cancer Res,2004,64(1): 189-195.
  • 6Farley J, Uyehara C, Hashiro G, et al. Cyclooxygenase-2 expression predicts recurrence of cervical dysplasia following loop electrosurgical excision procedure.Gynecol Oncol,2004,92(2):596-602.
  • 7Kim HJ, Wu HG, Park IA, et al. High cyclooxygenase-2 expression is related with distant metastasis in cervical cancer treated with radiotherapy.Int J Radiat Oncol Biol Phys,2003, 55(1):16-20.
  • 8Ishiko O, Sumi T, Yoshida H, et al. Association between overexpression of cyclooxygenase-2 and suppression of apoptosis in advanced cancer of the uterine cervix after cyclic balloon-occluded arterial infusion.Oncol Rep,2001,8(6): 1259-1263.
  • 9Gaffney DK, Holden J, Zempolich K,et al.Elevated COX-2 expression in cervical carcinoma:reduced cause-specific survival and pelvic control.Am J Clin Oncol,2001, 24(5):443-446.
  • 10Ferrandina G,Lauriola L,Zannoni GF, et al.Expression of cyclooxygenase-2 (COX-2) in tumour and stroma compartments in cervical cancer:clinical implications.Br J Cancer,2002,87(10):1145-1152.
苏州大学学报（医学版）
2008年 第1期

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