去卵巢和激素致骨质疏松模型中骨密度与血清指标的比较

The Analysis of Bone Mineral Density and Serum Index between Osteoporosis Models in Ovariectomized and Hormone

目的通过建立去卵巢与地塞米松致SD大鼠骨质疏松模型,了解去卵巢和地塞米松对骨代谢的影响,探讨两种模型在不同时间点骨密度和血清学的区别。方法选用6月龄健康雌性SD大鼠,采用肌注地塞米松及手术方法切除大鼠两侧卵巢分别制作去卵巢(OVX)及激素致大鼠骨质疏松模型。比较两者不同时间点血钙、磷、碱性磷酸酶(ALP)、骨密度、骨结构、骨组织形态学的变化。结果去卵巢组与地塞米松组大鼠的骨组织形态学都呈现出典型的骨质疏松状态,在各个时间点的骨密度均明显低于对照组(P<0.05或P<0.01)。另外在不同时间点两组模型大鼠体内ALP水平均显著升高(P<0.01),去卵巢组血钙、磷维持在正常范围(P>0.05),而地塞米松组钙降低(P<0.05),磷升高(P<0.05)。结论去卵巢与地塞米松所致的骨质疏松模型在不同时间点上的血清学指标、骨形态计量学存在一定的区别。同时发现去卵巢模型的造模成功时间可以提前到10周,而激素模型的造模时间则应为5周或更长。

Objective To establish osteoporosis models in ovariectomized(OVX) and dexamethasoned(Dex) SD rats,in order to understand OVX and Dex influence on bone metabolism.Exploring at different time points,the distinction of bone mineral density(BMD) and serologic between the two models.Methods Six months pure line female rats were chosen and were administrated i.m Dex.And the other rats were subjected to excise bilateal OVX.So that the two different osteoporosis models of rats were established.At different time po...