同种异体骨髓基质细胞移植修复兔膝关节骨软骨缺损

Repair of Articular Cartilage Defects with Allogeneic Bone Marrow Stromal Cells in Rabbit Knee Joint

目的探讨同种异体骨髓基质细胞(BMSCs)作为种子细胞在软骨组织工程中的应用。方法同种异体BMSCs与丝素蛋白-异体松质骨共培养构建组织工程化骨软骨复合体,移植修复兔膝关节骨软骨缺损,术后2、4、12周取材,行组织学观察及Ⅱ型胶原、Brdu免疫组化染色。结果BMSCs能在丝素蛋白和松质骨支架上较好的黏附、生长,共培养后成功地构建了组织工程化骨软骨复合体;体内移植后生成透明软骨样组织并修复了骨软骨缺损。结论同种异体BMSCs可作为软骨组织工程的种子细胞,丝素蛋白-松质骨双相支架负载同种异体BMSCs可用于骨软骨缺损的修复。

Objective To explore the possibility of use of allogeneic bone marrow stromal cells(BMSCs)in cartilage tissue engineering as seed cell.Methods A "two-phase" scaffold consisting of silk fibroin and allogeneic bone and BrdU labeled allogeneic bone marrow stromal cells were used to construct artificial engineered cartilage,and was implanted to repair bone and cartilage defects.The obtained specimens were examined histologically and stained immunohistochemically for type Ⅱ collagen and BrdU.Results BMSCs were a...