Resistin基因3’非翻译区ATG序列多态性与2型糖尿病的相关性研究

Study on the Association of the ATG Repeat in the 3'-Untranslated Region of the Human Resistin Gene and Type 2 Diabetes Mellitus

目的研究resistin基因3'-非翻译区ATG重复序列在2型糖尿病(T2DM)患者及正常血糖人群中的多态性分布,探讨此重复序列与T2DM的相关性。方法选取东北地区汉族T2DM患者243例,正常对照110例,采用聚合酶链反应-单链构象多态性(PCR-SSCP)方法初筛基因后,进行DNA直接测序。结果在353例受试者中,检测到resistin基因3’非翻译区ATG重复序列有3种基因型,表现为T2DM患者中a型ATG序列重复6次,共有220例,频率为90．5％;b型ATG序列重复8次,共有10例,频率为4．1％;c型ATG序列重复7次,共有13例,频率为5．4％。在110例正常人中均表现为重复6次的a型ATG序列,不存在b型及c型的基因型。结论Resistin基因3’非翻译区ATG序列在T2DM患者及正常人群中的多态性分布存在差异,其多态性分布与T2DM的易感性相关,可能是东北地区汉族人群发生T2DM的一个重要的相关因素。

Objective To study the polymorphism distribution of ATG repeats in the resistin gene 3 -UTR in T2DM and normal populations to examine whether the polymorphism are associated with T2DM. Methods 243 subjects with T2DM and 110 controls were randomly selected from Han Population in northeast China. Sequence variants of the resistin gene were analyzed with the Single Strand Conformation Polymorphism Analysis of Polymerase Chain Reaction Products (PCR-SSCP) technique as primary screening and then DNA direct seque...