三态MVR-PCR方法检测中国汉族人群D2S44(YNH24)基因座3’端遗传多态性

Three-State MVR-PCR: Studies of Genetic Polymorphism at 3' End of D2S44 ( YNH24) Locus in Chinese Han Population

目的揭示中国汉族人群(河北)D2S44基因座3’端的遗传多态性。方法采用三态MVR-PCR和变性PAGE-银染技术对D2S44基因座进行检测,结果用数字编码表示。结果在被检116名无关个体中,每个个体平均得到11个数字编码,3种重复单位出现的频率分别为:I型34．33％、Ⅱ型33．52％、Ⅲ型21．63％、0型10．52％。在116名无关个体中,两个无关个体拥有相同的编码概率为0．136 5,所有编码均相同的概率为3．06×1-10,杂合度为0．975 5,多态信息含量为0．975 1,非父排除率为0．951 0。结论D2S44基因座在中国汉族人群(河北)中具有较高的遗传多态性,是法医学和人类群体遗传学研究的一个重要遗传标记。

Objective To reveal the polymorphism at 3 end of D2S44( YNH2A) locus In Chinese Han population. Methods Three-state MVR-PCR ( minisatellite variant repeat by PCR) was used to amplifiy D2S44 fragment. PCR products were separated on vertical, denaturing, polyacrylamide gels followed by silver staining and digital codes were obtained. Results In a total of 116 healthy unrelated individuals investgated, about 11 digital codes were obtained from each individual. The frequency of type- I , type-II , type-III and...