加拿大一枝黄花与“黄莺”的ITS同源性分析

Phylogenetic relationship of Solidago canadensis L.and huangying based on ITS

根据一枝黄花属核糖体基因转录间隔区(ITS)序列设计引物Y2/Y4,分别扩增了加拿大一枝黄花和“黄莺”的ITS区段,并对PCR产物进行了序列测定和分析。扩增的序列长度为596bp。序列分析的结果表明“黄莺”与加拿大一枝黄花属于同一聚类组,同源性为99.8%~100%,亲缘关系极近。

rDNA ITS region of Solidago L.was amplified with primers Y2/Y4 deriving from ITS of Genus Solidago.Phylogenetic relationship of Solidago canadensis L.and huangying were analysized based on the rDNA ITS squences by the software “DNASTAR”. Solidago canadensis L.and huangying were included in the same group with similarity of 99.8%~100%,so they were nearest in all research samples.