利用分子生物技术对厌氧颗粒污泥中微生物种群结构的研究

Study on microbial community in anaerobic granular sludge by molecular technology

利用F ISH、RTQ-PCR和DGGE等分子生物技术对厌氧产甲烷颗粒污泥中微生物种群的多样性、空间分布和定量关系进行研究,并对其中的优势古细菌进行系统发育分析。结果表明:颗粒污泥中真细菌主要分布在颗粒污泥外层,古细菌则主要分布在内层;古细菌含量低于真细菌,但有逐渐增多的趋势;随着反应器有机负荷的增加以及运行时间的延长,真细菌种群结构相对稳定,而古细菌种群结构则发生了较明显变化,其中占优势的古细菌种类逐渐减少,主要包括甲烷微粒菌(M ethanocorpuscu lum)、甲烷杆菌(M ethanobacter ium)和甲烷髦毛菌(M ethanosaeta)等。

The biodiversity,spatial distribution and quantification of different microbial species in the anaerobic methanogenic granules were investigated by FISH,RTQ-PCR,DGGE,and the phylogenetic analysis of dominant archaea were conducted.The results indicated that: most eubacteria were located in the surface area of the(granule),while most archaea were located in the inner layer;the quantity of archaea was less than eubacteria,but increased gradually;as the organic loading rate of the reactor increased and the ope...