摘要
基因突变的检测在临床疾病诊断中起十分重要的作用.单链构象多态性(Single Strand Conform ationPolym orphism,SSCP)分析是检测突变最流行的方法之一.SSCP分析与毛细管电泳(Capillary Electrophoresis,CE)相结合的技术更是具有灵敏度高、花费低、简单、快速的优点.目前,这项技术已应用于人类原癌基因、抑癌基因以及其它致病基因的突变检测.主要综述了各种参数对CES-SCP分析的影响以及CES-SCP分析技术将来的发展方向.
Mutation detection plays an important role in clinical diagnosis of diseases.Single strand conformation polymorphism(SSCP) analysis is one of the most popular methods for detection of mutations.SSCP analysis in combination with CE is a rapid,simple,sensitive and low-cost tool,and has been successfully applied for mutation detection involving human tumor oncogenes suppressor genes and disease-causing genes.Basic issues about the influence of parametes for SSCP analysis based on CE are reviewed and the prospe...
出处
《生命科学研究》
CAS
CSCD
2006年第S1期10-14,共5页
Life Science Research
基金
甘肃省自然基金资助项目(ZS031A-25-70E-)
中国博士后基金资助项目(2005037576)
关键词
毛细管电泳
单链构象多态性
荧光标记
筛分介质
毛细管涂层
capillary electrophoresis
single strand conformation polymorphism
fluorescent labeled
sieving matrices
capillary coating
