摘要
目的探讨低拷贝模板(low copy number,LCN)STR扩增方法,提高LCN检材的检验成功率。方法采用Profiler P lusTM试剂盒与9947A对照DNA,改变Taq酶量、体系、循环次数3个因素进行扩增检验,了解各变量对扩增检测的影响。结果对低拷贝模板DNA,单纯增加Taq酶量或反应体系,扩增效率改善不明显;增加循环数,显著提高检验灵敏度;低于0.01ng的模板DNA,同时增加扩增体系、Taq酶量、循环数在一定程度上提高扩增效率。结论对于影响扩增的Taq酶量、体系、循环次数3个因素中,循环数影响最大,但应慎用34次及以上循环数;三者同时增加,对于低于0.01ng模板DNA的扩增可有效改善。
Objective To explore the methods to amplify LCN-STR,and improve the success rate of LCN-STR typing.Methods Using the Profiler Plus^(TM) kits and 9947A control DNA,the effects of three factors in PCR on the results of the STR amplification were observed through changing the amount of Taq enzyme,reaction volume and PCR cycles.Results For LCN template DNA,if only increased the amount of Taq enzyme or the reaction volume,the results of STR amplification were scarcely improved,but if increased PCR cycles,the det...
出处
《中国法医学杂志》
CSCD
2006年第S1期4-6,2,共4页
Chinese Journal of Forensic Medicine
关键词
法医物证学
低拷贝模板
STR分型
Taq酶量
扩增体系
循环次数
Forensic biological evidence
Low copy number（LCN）
STR genotyping
Taq enzyme
Amplification reaction volume
PCR cycles
