熊胆对肿瘤细胞p53蛋白表达的影响

Effects of Bears' Bile on the Expression of Tumor Cell p53 Protein

目的探讨熊胆对p53基因特性不同的肿瘤细胞p53蛋白表达的影响。方法采用Western blot和RT-PCR分析方法检测熊胆对6个癌细胞株p53蛋白表达影响。结果Western blot分析可见,有p53基因突变的HaCaT、KUMA3、KUMA4和KUMA6细胞株p53蛋白表达增加,而无p53基因突变的HCT116和KUMA5细胞株p53蛋白表达没有变化。经RT-PCR分析p53mRNA结果,所有细胞株都没有p53mRNA量的变化。结论熊胆对癌细胞株p53蛋白表达的影响是依其所具有的p53基因特性不同而不同的,即熊胆只作用于突变型p53蛋白。

Objective To explore the effects of bears bile on tumor cell p53 protein expression with differ- ent gene properties.Methods The effects of bears bile on the expression of p53 protein in 6 cancer cell strains were determined by Western blot and reverse transcription-polymerase chain reaction(RT-PCR)analysis.Results Western blot analysis showed that the expression of p53 protein in HaCaT,KUMA3,KUMA4 and KUMA6 cell strains with gene mutation were increased,but no change was found in HCT116 and KUMA5 cell stra...