TC指数在改良LONGA线栓法局灶性脑缺血/再灌注动物模型制备中的应用

Application of TC Index Location on LONGA's Animal Model of Regional Experimental Cerebral Ischemia and Reperfusion

目的观察TC指数定位血管线栓法对大鼠局灶性脑缺血模型制备的影响。方法300只雄性SD大鼠随机分为预试验组30只、原始方法组120只(按LONGA氏血管线栓法)、改良方法组150只(按动物门齿根部T点距颈总动脉分叉点C点长短而定,简称TC指数定位法)；比较两种不同造模法对血管线栓局灶性脑缺血/再灌注实验大鼠的成功率及形态学的变化。结果TC指数定位法改良的LONGA氏血管线栓再灌注制模法,比原始的LONGA氏血管线栓法大鼠脑缺血/再灌注模型成功率高,直线相关分析及统计学上均具有显著性意义；形态学表现差异无显著性。结论TC指数定位法的LONGA氏血管线栓法大鼠脑缺血/再灌注模型是一种较好的制模方法,值得实验研究中推广应用。

Objective To study the TC index location on a reform LONGA s animal model of regional exper- imental cerebral ischemia and reperfusion.Methods A total of 300 SD male rats were randomly divided into the pre-test group (n=30),the original method group (n=120,Longa s method) and the reform method group [n= 150,TC index location is defined by the length of rat incisor teeth root (T point) to the common carotid artery bi- furcation (C point),the blood flow of a unilateral middle cerebral artery in the rats was r...