摘要
目的研究哮喘大鼠肺组织中白三烯B_4(LTB_4)、白三烯C_4(LTC_4)及白三烯D_4(LTD_4)含量的变化及中药复方双龙胶囊对其的影响。方法将SD大鼠随机分成空白组、哮喘模型组、对照组(地塞米松组)、高、中、低3个中药剂量组。用卵蛋白致敏大鼠,以抗原攻击后建立哮喘模型,并用反向高效液相色谱法(RP-HPLC)测定各组大鼠肺组织匀浆中LTB_4、LTC_4、LTD_4含量,并进行比较。结果哮喘大鼠肺组织中LTB_4、LTC_4、LTD_4含量较正常对照组比均有显著升高,地塞米松和双龙胶囊8．27 g/kg,4．13 g/kg均可明显抑制哮喘大鼠肺组织中白三烯的生成(P＜0．05)。结论双龙胶囊可明显抑制哮喘大鼠肺组织中炎性介质白三烯的生成,这可能是双龙胶囊用于治疗支气管哮喘的抗炎平喘作用机制之一。
Objective To observe the changing contents of leukotriene B_4 (LTB_4),leukotriene C_4 (LTC|_4), and leukotriene D_4 (LTD_4) of lung tissue in asthmatic rats,and explore the effect of Shuanglong Capsule (SLC) on it.Methods SD rats were randomly divided into the nomal group,asthmatic model group,Dexamethasone group and the high,middle and low dose SLC groups.All rats except those in the normal group were sensitized by ovalbumin and challenged with the antigen,and the contents of LTB_4,LTC_4 and LTD_4 in lung ...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2006年第S1期43-46,共4页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
支气管哮喘
白三烯
高效液相色谱法
双龙胶囊
bronchial asthma
leukotriene
high performance liquid chromatography
Shuanglong Capsule
