摘要
目的探讨中药草果知母汤抗痫作用的受体机制。方法中药组SD大鼠连续给予24天的草果知母汤灌胃,对照组给予相应体积的生理盐水。比较中药组与对照组戊四唑惊厥闲值的差异。运用免疫印记及免疫组化法定量定位检测大鼠脑海马中γ-氨基丁酸A受体α_1亚单位(GABA_AR-α_1)和N-甲基-D-天冬氨酸受体1亚单位(NMDAR-NR_1)蛋白表达的变化；运用受体配基结合实验比较脑海马中两个受体结合的改变。结果中药组GABA_AR-α_1亚单位蛋白表达明显高于对照组；而NR_1亚单位蛋白表达则与对照组的差异无显著性。GABA_A受体的结合同对照组比较也明显升高,而NMDA受体结合则无明显变化。结论草果知母汤通过升高GABA_A受体α_1亚单位蛋白的表达,以及与配基结合的增强而起到抗癫痫的作用。
Objective To study the receptor mechanism of anti-convulsant effect of Caoguo Zhimu Decoction (CZD).Methods SD rats in traditional Chinese medicinal (TCM) group were given CZD by gastrogavage for 24 days,while those in the control group were given normal saline of the same volume.The difference of convulsant threshold was compared between the TCM group and the control group induced by pentylenetetrazole.Changes of gamma-aminobutyrie acid A receptor (GABA_AR-α_1)and N-methyl-D-aspartate receptor-NR_1(NMDAR-N...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2006年第S1期36-38,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助(No.30440076)
