儿童非霍奇金淋巴瘤MDM2和p53蛋白表达的研究

Relationship between the expression of MDM2 protein, p53 protein and non-Hodgkin lymphoma in childhood

目的探讨儿童非霍奇金淋巴瘤(NHL)患者癌基因MDM2和抑癌基因p53的表达与NHL的恶性程度和预后的关系。方法46例儿童NHL患者作为病例组,12例儿童淋巴结炎患者作为对照组。用免疫组织化学超敏S-P染色法,检测病例组NHL患者、对照组淋巴结炎患者病理组织的MDM2蛋白和p53蛋白的表达情况,阳性细胞为胞核染成黄色￣棕黄色,阳性细胞数≥10%为MDM2免疫组织化学阳性(MDM2蛋白过度表达);阳性细胞数>5%为p53免疫组织化学阳性。结果病例组MDM2蛋白过度表达率为65.2%,与对照组比较差异有统计学意义(P<0.01)。p53蛋白阳性率为19.6%,与对照组比较差异无统计学意义(P>0.05)。MDM2蛋白过度表达率在病理分类高度恶性、患者血清LDH增高之间差异均有统计学意义(P<0.05),与患者的B状态间差异有统计学意义(P<0.01)。p53蛋白阳性率在病理工作分类、临床分期、B状态、血清LDH之间差异均无统计学意义(P>0.05)。MDM2蛋白过度表达与p53蛋白阳性之间没有关联性。结论儿童NHL患者MDM2基因的过度表达率较高;MDM2过度表达与儿童NHL患者的不良状况、不良预后有关;儿童NHL患者p53阳性率较低。

Objective To investigate the relationship between the expression of MDM2 protein, p53 protein and the malignant grade as well as prognosis of non-Hodgkin lymphoma(NHL) in childhood. Methods 46 cases of NHL were chosen as patient group and 12 cases of lymphadenitis as control group. Immunohistochmistry ultrasensitive S-P assay was used to detect the expression of MDM2 protein and p53 protein in pathological tissues in all cases. Positive cells were dyed yellow or brown in nuclei. Positive MDM2 was that the p...