挥发性有机化合物致NIH/3T3细胞形态转化的作用

Morphological Transformation of Mouse NIH/3T3 Cells Induced by Volative Organic Compounds in vitro

目的探讨挥发性有机化合物(VOCs)中两种主要成份苯、甲醛的联合致细胞转化活性。方法采用细胞灶法观察甲醛与苯联合致小鼠NIH/3T3细胞的形态学转化作用。结果苯、甲醛及苯和甲醛联合染毒组均有细胞转化灶形成,其形成率均呈剂量依赖关系。联合作用分析显示:苯25μg/mL+甲醛25μg/mL,苯25μg/mL+甲醛50μg/mL,苯50μg/mL+甲醛25μg/mL,苯50μg/mL+甲醛50μg/mL,两化合物联合作用转化率实测值分别为3.6%、5.8%、5.7%和7.6%。而预期转化率分别为3.5%、5.6%、5.6%、7.7%。说明在等剂量原则下,苯、甲醛单独染毒的转化细胞灶形成率之和与两化合物同时染毒的转化细胞灶形成率非常接近;析因分析表明两者间无交互作用(F=0.07,P>0.05)。结论苯、甲醛具有细胞转化活性,二者联合作用类型呈相加作用。

Objective To investigate the role of morphological transformation of benzene combined with formaldehyde in virto.Method Induction of cell morphological transformation by the combination of benzene and formaldehyde was performed.Results Transformed foci were induced by benzene,formaldehyde and their combination.The transformation frequencies were dose-dependent.Transformation frequencies by simultaneous treatments of benzene and formaldehyde at dosage of 25+25μg/mL,25+50μg/mL,50+25μg/mL and 50+50μg/mL were 3...