甘蓝品种的AFLP指纹鉴别图谱分析

Analysis of AFLP Fingerprinting in Cabbage Cultivars Testing

本试验采用AFLP技术分析了来自全国9个栽培地区的44个甘蓝主栽品种,共筛选了40对E+3/M+3引物组合,多态性条带的数量从0条到15条不等。其中引物组合E-AAC/M-CTA是甘蓝品种中多态性最高的引物,有15条多态性条带,多态性条带的百分率为30%,但该引物不足以区分44个供试的甘蓝品种。同时筛选了11组E+2/M+3对引物组合,其中引物组合E-AG/M-CTC产生了13条清晰的多态性条带。以E-AAC/M-CTA和E-AG/M-CTC这两对引物组合的多态性条带构建了44份甘蓝品种材料的指纹图谱,此指纹图谱可以将供试的44份材料一一区分。

In this study, AFLP was used to evaluate the genetic polymorphism of cabbage cultivars, including 44 cultivars collected from 9 different culture regions. 40 pairs of E+3/M+3 AFLP primers were screened. The range of polymorphic bands detected by individual pair of primer were from 0 to 15. E-AAC/M-CTA which detected 50 fragments has particularly high efficiency detecting 15 polymorphic fragments (30%), but it was not able to distinguish all of the 44 accessions. Then 11 pairs of E+2/M+3 AFLP primers were screened. E-AG/M-CTC detected 13 polymorphic fragments. 44 cabbage cultivars testing fingerprinting has been established with these polymorphic bands combined E-AAC/M-CTA and E-AG/M-CTC, which was able to distinguish all of the 44 accessions.