生殖器疱疹患者外周血CD14^+单核细胞Toll样受体的表达及其意义

Expressions of Toll-like Receptors on Peripheral Blood CD14^+ Monocyte in Patients with Genital Herpes

目的:探讨CD14+单核细胞Toll样受体(TLRs)的表达在复发性生殖器疱疹(RGH)发病机制中的作用。方法:采用双色免疫荧光抗体染色流式细胞术检测了34例RGH患者外周血单核细胞表面TLR4、TLR1、TLR2的表达水平,并以36例正常人作为对照。结果:RGH患者外周血单核细胞TLR4的表达水平显著高于对照组(P<0.001),TLR1的表达水平则比对照组明显降低(P<0.001),TLR2的表达水平在以上两组中差异无统计学意义(P>0.05))。结论:单纯疱疹病毒感染能上调单核细胞TLR4的表达,在病毒识别、增强信号传导、启动抗病毒的固有免疫反应中起着重要作用。

Objective:To explore the expressions of Toll-like receptors(TLRs) on CD14^+ monocytes and the role in the pathogenesis of recurrent genital herpes(RGH). Methods:The expressions of TLR4,TLR1 and TLR2 on peripheral blood CD14^+monocytes were analyzed by flow(cytometry) with two-colour immunofluorescent staining in 34 patients with RGH and 36 healthy controls.Results: The expression of TLR4 on CD14^+monocytes was significantly higher in patients with RGH than that in healthy controls(P<0.001),but TLR1 expressed on monocytes from RGH patients was markedly lower than that in controls(P<0.001).There was no significant difference in expression of TLR2 between patients and controls(P>0.05).Conclusion:The expression of TLR4 on monocytes could be upregulated by herpes simplex virus infection,which may play a key role in recognizing virus,enhancing signal transduction and initiating innate immune responses to viral pathogens.