白藜芦醇预处理对心肌缺血/再灌注损伤的保护作用

Protective effect of resvaratrol pretreatment on myocardial ischemic/reperfusion induced injury in rats

目的:观察白藜芦醇预处理对心肌缺血再灌注损伤的保护作用。方法:45只雄性SD大鼠随机分为假手术组、缺血再灌组及白藜芦醇预处理组,结扎左冠状动脉制作心肌缺血再灌注模型,比较各组左室最大收缩压(LVSP)、左室等容收缩/舒张期压力上升最大速率(LVdP/dtmax),心肌一氧化氮(NO)、丙二醛(MDA)含量变化及心肌梗死范围。结果:白藜芦醇预处理组LVSP、LVdP/dtmax较缺血再灌组显著升高,心肌NO含量显著升高、MDA含量则显著降低,心肌梗死范围明显减小。结论:白藜芦醇预处理对心肌缺血再灌注损伤有明显保护作用,其机制与白藜芦醇抗氧化、清除自由基及增加NO合成有关。

Objective:To study the protective effect of resveratrol pretreatment on myocardial ischemia/reperfusion(I/R) induced injury.Methods: Male Sprague-Dawley rats were randomly devided into three groups:sham group?control group and resveratrol pretreatment group. I/R models were prepared by a temporary occlusion of the left anterior descending coronary artery. Results:Resvaratrol improved postreperfusion cardiac function by increasing left ventricle maximum systolic pressures (LVSP) and the maximal first derivative of developed pressure (dP/dtmax), shrinked the size of myocardial infarction, decreased myocardial malonaldehyde (MDA) content and increased myocardial nitric oxide (NO) content. Conclusion: Resveratrol has protective action on myocardial I/R induced injury in rats. The cardioprotective effects in the I/R rats may be correlated with its antioxidant activity and upregulation of NO production.