徐州市急性呼吸道感染儿童中人偏肺病毒感染状况研究

Study on Infection Status of Human Metapneumovirus in Children of Xuzhou City

目的了解徐州市因急性呼吸道感染而就诊的儿童中hMPV感染情况,并简要描述病毒感染后引起的临床症状。方法采集咽拭子,提取RNA,用设计的引物进行一步法RT-PCR,扩增产物用琼脂糖凝胶电泳检测。结果83份咽拭子标本,21份标本为hMPV的RNA阳性标本,感染率25·30%。阳性患者中80·95%表现为高热(≥38·5℃)、咳嗽,28·57%缺氧,61·90%患者被诊断为支气管炎。结论徐州地区儿童中的急性呼吸道感染与hMPV感染有关;咳嗽、发热是hMPV感染后的主要临床症状。

Objetive To understand the infection status and clinical symptoms of human metapneumovirus in children of Xuzhou City. Methods RNA were extracted from the throat swab and amplified by 1-step reverse transcription-polymerase chain reaction. Results Among 83 patients, 21 (25.30%)had evidences of hMPV infection. Clinical symptoms included cough, fever and hypoxia. The most frequent diagnoses of children were Bronchitis. Conclusions The acute respiratory-tract infections among children of Xuzhou city are associated with hMPV. Cough and fever are major clinical symptoms.