栀子中三萜酸的提取及结构鉴定

Study on Isolation and Identification of Triterpene Acid from Gardenia jasminodes Eills

对栀子中的主要药用成分三萜酸进行了提取及纯化研究,用95%乙醇,固液比1∶8(kg∶L),在85.4℃回流提取3次,每次2.5h,浸出率达92.6%。通过DSC、IR、EI-MS、1H和13CNMR、HPLC等现代分析手段对提取物进行了结构鉴定,证实其为熊果酸,平均含量为0.4445%。

Isolation and purification of triterpene acid from fruit of Gardenia Jasminodes Eills were studied,the optimum processing conditions are: the granularity of powder 0.4 mm, ratio of liquid to solid 8∶1, temperature 85 ℃, 3 times of extraction of 2.5h each, and concentration of alcohol 95 %.Extraction rate of ursolic lixiviate is 92.6 %. Structural identifications by DSC,IR, EI-MS ,~1H and ~ 13 C NMR,HPLC show that the triterpene acid is ursolic acid,the average content of ursolic acid is 0.444 5 %。