二倍体鸭茅染色体加倍的研究

Studies on Chromosome Doubling with Diploid Dactylis glomerata

研究了秋水仙碱不同浓度(0.05%、0.2%、0.3%)、不同处理时间(1d、2d、3d)、种子不同萌芽阶段(芽长<0.5cm、1～2cm、>3cm)对二倍体鸭茅染色体加倍效果及生长发育的影响.结果表明,三个因素对诱变效果均有一定程度的影响,所有染色体加倍植株均为混倍体,即同一根尖细胞中二倍体和四倍体细胞共存;混倍体早期生长弱于二倍体,以后的生长发育及开花期形态特征二者几乎无差异.

Effects of chromosome doubling,growth and development of diploid Dactylis glomerata were studied based on the change of colchicines concentration(0.05%,0.2%,0.3%),treatment time(1d,2d,3d) and the length of bud(0.5cm、1～2cm、>3cm).The results showed that:(1) Three factors have some effects on the mutagenic results;(2) All seedlings with doubled chromosome number were mixed-ploids,diploids(2n=14) and tetraploids(2n=28) cells are all existed in a root tip cells;(3) The early growth of the mixed-ploids was weaker than diploids,but little difference existed in latter growth,development and morphological characters in flowering stages.