分子蒸馏和脂肪酶催化水解菜籽油脱臭馏出物浓缩维生素E的优化研究

Molecular distillation and enzymatic hydrolysis for concentrating vitamin E from rapeseed oil deodorizer distillate

采用分子蒸馏耦合脂肪酶催化水解菜籽油脱臭馏出物,并用碱中和、水洗萃取以浓缩维生素E。着重考察了脂肪酶活力,添加的水量和反应时间对水解效果的影响。通过正交实验获得较好的水解工艺条件:添加的水量/水解原料比15 mL/15 g,脂肪酶活/水解原料105 U/g,反应时间5 h,反应温度37℃,经后续处理后维生素E纯度达到17.3%,回收率85%。固定化酶可以重复利用多次。

The molecular distillation and enzymatic hydrolysis were used for concentration of vitamin E from rapeseed oil deodorizer distillate.Free fatty acids liberated were neutralized using alkali and the salts formed then washed off.Factors affecting the degree of hydrolysis were studied to reach better recovery of vitamin E by orthogonal experiment.The results indicated that the content and recovery of vitamin E were 17.3% and 85% respectively when water/material is 15∶15 mL/g with 105 U/g of lipase activity,and reacting for 5 h at a temperature of 37℃.And the immobilized lipase can be used for many times in hydrolysis.