人乳头瘤病毒感染基因亚型分析

Genotyping analysis in human papillomavirus infection

目的分析HPV各基因亚型感染情况,探讨HPV亚型感染与宫颈病变的关系。方法应用美国Digene公司杂交捕获法(Hybrid Capture Ⅱ,HC Ⅱ)对我院宫颈疾病专科门诊病例进行高危型HPV筛查,从中随机抽取213例阳性标本应用PCR结合反向寡核苷酸探针斑点杂交技术(reverse dot blot,RDB)进行HPV基因亚型分析。结果213例HCⅡ法检测为高危型HPV阳性的标本中被PCR/RDB法检测出具体HPV高危型别的有195例。共检测出17种HPV型别(包括高危型13种和低危型4种)。具体HPV亚型检出率分别是16型(32.3%)、52型(23.1%)、58型(18.0%)、31型(11.8%)、68型(10.3%)、33型(9.7%)、53型(8.2%)、18型(8.2%)、66型(6.2%)、CP8304型(4.6%)、6型(3.6%)、59型(3.1%)、45型(2.1%)、39型(2.1%)、11型(2.1%)、56型(1.5%)、51型(1.0%)。单一HPV亚型感染及2种、3种和4种HPV亚型混合感染检出率分别为59.5%、30.8%、8.6%和1.1%。结论高危型HPV感染检出率最高的HPV亚型为HPV16型(32.3%),其次为HPV52型(23.1%),检出率最低的两种亚型分别是HPV56型(1.5%)和HPV51型(1.0%)。HPV58型这种世界范围内少见的一种高危型HPV在中国高危型HPV感染的患者中检出率(18.0%)却并不低。单一高危型HPV亚型感染是引起宫颈病变的主要原因,而多种HPV亚型混合感染在宫颈病变中的作用也不可忽视。

Objective Analyse the genotypes of human papillomavirus in clinical cases with papillomavirus infection. Methods 213 high risk HPV positive samples screened by Digene Hybrid Capture II were genotyped by PCR/Reversed dot blot hybridization. Results 195 high risk HPV positive cases could be genotyped specifically by PCR/RDB. 17 high risk and low risk HPV genotypes were detected, namely HPV 16(32.3%)、HPV 52(23.1%)、HPV 58(18.0%)、HPV 31(11.8%)、HPV 68(10.3%)、HPV 33(9.7%)、HPV 53(8.2%)、 HPV 18(8.2%)、HPV 66(6.2%)、HPV CP8304(4.6%)、HPV6(3.6%)、HPV59(3.1%) HPV45(2.1%)、HPV39(2.1%)、HPV11(2.1%)、HPV56(1.5%)、HPV51(1.0%). Among the 195 HPV infected samples, the detection rates of 1 HPV genotype infection, 2 HPV genotypes infection, 3 and 4 HPV genotypes infection were 59.5%, 30.8%, 8.6%, 1.1%, respectively. Conclusion The major genotypic prevalence in high risk HPV infection was HPV 16, HPV 52 and HPV 58. The HPV 56 and HPV 51 had less detection rate. HPV 58 had lower detection rate in other parts of the world whereas there was high incidence in China. Single HPV genotype was key HPV infection. Mixed infection of more than two HPV genotypes should be paid more attention.