摘要
Objective:To investigate the chemical constituents of Polygala japonica Houtt.. Methods:The constituents were separated and purified by column chromatographies with silical gel, RP-silical gel and Sephadex LH-20. Their structures were elucidated on the basis of spectral data (IR, MS, 1H-NMR, 13C-NMR, HMQC and HMBC). Results: Totally 15 compounds were obtained from Polygala japonica Houtt. And their structures were identified as castaprenol-12 (Ⅰ), β-sitosterol (Ⅱ), stigmasterol (Ⅲ),stigmasta-7,22-di-ene-3-one (Ⅳ), n-dotriacontanol ( Ⅴ ), n-hexadecanol ( Ⅵ ), arachidic acid (Ⅶ), erogosterol (Ⅷ), kaempferol (Ⅸ), quercetin (Ⅹ), daucosterol (Ⅺ), p-hydroxybenzoic acid (Ⅻ),coumaric acid (ⅩⅢ), rhamnocitrin 3-O-β-D-galactopyranoside (ⅪⅤ), quercetin 3-O-β-D-glucopyranoside (ⅩⅤ). Conclusion: Compounds Ⅰ , Ⅲ , Ⅳ, Ⅴ , Ⅵ, Ⅶ, Ⅷ, Ⅻ , ⅩⅢ and ⅩⅣ were obtained from this plant for the first time.
Objective: To investigate the chemical constituents of Polygala japonicaHoutt.. Methods: The constituents were separated and purified by column chromatographies withsilical gel, RP-silical gel and Sephadex LH-20. Their structures were elucidated on the basis ofspectral data (IR, MS, ~1H-NMR, ^(13)C-NMR, HMQC and HMBC). Results: Totally 15 compounds wereobtained from Polygala japonica Houtt. and their structures were identified as castaprenol-12 ( Ⅰ), β-sitosterol ( Ⅱ ), stigmasterol ( Ⅲ ), stigmasta-7,22-di-ene-3-one (Ⅳ), n-dotriacontanol(Ⅴ), n-hexadecanol (Ⅵ), arachidic acid (Ⅶ), ero-gosterol (Ⅷ), kaempferol (Ⅸ), quercetin (Ⅹ),daucosterol (Ⅺ), p-hydroxybenzoic acid (Ⅻ), coumaric acid (XIII), rhamnocitrin3-O-β-D-galactopyranoside (XIV), quercetin 3-O-β-D-glucopyranoside (XV). Conclusion: Compounds Ⅰ, Ⅲ , Ⅳ , Ⅴ , Ⅵ , Ⅶ , Ⅷ , Ⅻ , XIII and XIV were obtained from this plant for the first time.
基金
Supported by the 863 Foundation of China ( No.2003AA2Z3507 ) the Scientific Foundation of Shanghai,China (No. 03QMH1414, 04DZ19843, 04DZ19856 and 04DZ19857)
