乙型肝炎血清标志物与HBV DNA含量的相关性分析

Relationship between amount of HBV DNA in serum and HBV markers

目的探讨乙型肝炎病毒(HBV)DNA检测结果与血清标志物检测结果的相关性。方法采用荧光定量聚合酶链反应(FQ-PCR)和酶联免疫吸附(ELISA)法同步检测125份血清标本HBV DNA含量和HBV标志物。结果125份血清标本中,47例HBsAg(+)、HBeAg(+)、抗-HBc(+)患者血清HBV DNA检测阳性率为95.7%;35例HBsAg(+)、抗-HBe(+)、抗-HBc(+)患者血清中HBV DNA检测阳性率为65.7%,血清HBeAg阳性组HBV DNA检测含量明显高于HBeAg阴性组。结论结论FQ-PCR法检测HBV DNA具有良好的特异性和灵敏度,为临床了解病毒的复制情况,选择制定治疗方案的和疗效观察提供了有力的依据。

Objective To study the relationship between the amount of HBV DNA in the serum of patients and the HBV serological markers.Methods The amount of HBV DNA in the serum of 125 patients was detected with fluorescence quantitative polymerse chain reaction(FQ-PCR).At the same time, the serum HBV markers of these patients were detected with ELISA.Results In 47 samples with HBsAg(+),HBeAg(+),anti-HBc(+), the positive rate of FQ-PCR results was 95.7%. In 35 samples with HBsAg(+),anti-HBe(+),anti-HBc+,the positive rate was 65.7%.The concentration of HBV DNA in HBeAg-positive group was obviously higher than that in anti-HBe group.Conclusion FQ-PCR,used to detect the serum HBV DNA is very specific and sensitive. It provids definite clinical basis for studying the duplicative state of the hepatitis B virus ,marking therapeutic plans and observing the effect of therapy.