LH-750对有核红细胞分析的应用评价

Evaluation on clinical applications of automatic nucleated red blood cell count by LH-750 blood cell analyzer

目的评价COULTERLH-750血液学分析仪对有核红细胞分析结果的准确性。方法采用仪器法和手工法分别对120份外周血中含有有核红细胞的临床血液样本进行测定。结果仪器法和手工法对有核红细胞(NRBC)的检测结果比较,中、高值差异无统计学意义(P>0.05),低值差异有统计学意义(P<0.01)。标本重复性能良好,平均CV<6%,灵敏度92.3%,特异性97.7%,假阳性14.3%,假阴性1.16%。结论血液学分析仪LH-750对NRBC分析结果准确、快速,有助于一些血液病及肿瘤化疗患者的筛查与诊断。

Objective To evaluate the veracity of automatic nucleated red blood cell(NRBC)count by COULTER LH-750 blood cell analyzer.Methods The peripheral blood NRBC of 120 patients were counted by both COULTER LH-750 automatic blood cell analyzer and manual method with microscope.Results The counting results of high value and medium value had no significant difference between the manual and automatic methods(P> 0.05 ),the counting results of low value had significant difference between the manual and automatic methods(P<0.01).The average CV of absolute NRBC count of high,medium and low value was below 6%.Sensitivity was 92.3% ,specificity 97.7%,false positive 14.3%,false negative 1.16%.Conclusion The NRBC counting by the COULTER LH-750 blood cell analyzer is accurate,quick and helpful to the screening and diagnosis of some hematological and tumorous chemotherapy patients.